ACCPA (Human) ELISA Kit - E4965

E4965 | ACCPA (Human) ELISA Kit DataSheet

Anti-cyclic citrullinated peptide antibodies (ACCPA) are autoantibodies that target citrullinated peptides or proteins. Citrullination or deimination is a posttranslational modification wherein peptidyl arginine is converted to citrulline by peptidyl arginine deiminases (PADIs). Citrullination of proteins is observed in inflammatory diseases such as Alzheimer's disease, multiple sclerosis, rheumatoid arthritis, and cancers. In joints, the citrullination of proteins triggers an immune response that results in the formation of autoantibodies such as ACCPA against joint proteins. ACCPA constitutes about 60-80% of the serum of patients with rheumatoid arthritis (RA) and can help diagnose RA at an early stage. Hence, ACCPA is considered a diagnostic biomarker of rheumatoid arthritis. ’s ACCPA (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of ACCPA in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of ACCPA in the sample or standard.

Alternate Name : ACCPA, CCP, anti-CCP, ACPA, Cyclic Citrullinated Peptide

Tag Line : A Sandwich ELISA Kit for quantitative measurement of Anti-Cyclic Citrullinated Peptide Antibody (ACCPA) in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Other biological fluids Serum Tissue lysate Plasma

Species Reactivity : Human

Applications : Sandwich ELISA Kit to quantitatively measure ACCPA in human serum, plasma, tissue lysates and other biological fluids

Features and Benefits : Detection range: 1.563 – 100 ng/ml Recovery range: 85 - 105% for normal human serum and plasma samples Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10% This Sandwich ELISA is highly sensitive and highly specific for the detection of ACCPA in human samples. There is no significant cross-reactivity or interference between ACCPA and analogues Sensitivity: 0.938 ng/ml