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Aldose Reductase, human recombinant | 7361
- SKU:
- 26-7361-GEN
- Availability:
- Usually shipped in 5 working days
Description
Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E.coli and purified by using conventional chromatography techniques.
7361 | Aldose Reductase human recombinant DataSheet
Biomolecule/Target: Aldose Reductase
Synonyms: Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR
Alternates names: Aldo-keto reductase family1, member B1, AKR1B1, ADR, ALDR1, ALR2, AR
Taglines: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds
NCBI Gene ID #: 231
NCBI Gene Symbol: AKR1B1
Gene Source: Human
Accession #: P15121
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Specific activity is approximately 0.2-0.9 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/min at 25°C. Specific activity was expressed in units/mg of protein.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 35.8 kDa (316 aa, 1-316 aa), confirmed by MALDI-TOF.
Concentration: 1 mg/ml
Appearance: Liquid
Physical form description: 1 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT and 10% glycerol.
Reconstitution Instructions: N/A
Amino acid sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF