AMP Colorimetric Assay Kit - K229

K229 | AMP Colorimetric Assay Kit DataSheet

Adenosine Monophosphate (AMP), also known as 5’-adenylic acid, consists of adenine, ribose and phosphate. AMP can be produced during ATP synthesis by the enzyme adenylate kinase by combining two ADP molecules, as a result of the hydrolysis of ATP/ADP, or when RNA is broken down. AMP plays an important role in many cellular metabolic processes, such as Ca2+ signaling, cell migration, cytokine secretion etc. AMP is an activator of AMP-activated protein kinase, which regulates glucose uptake, fatty acid uptake, fatty acid oxidation etc. AMP levels can be measured by luciferase/luciferin mediated assays. However, luciferase signal is unstable and luminescent equipment is generally not available in most laboratories. ’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.

Alternate Name : AMP Assay Kit, AMP Colorimetric Kit, Adenosine Monophosphate Assay, Adenosine Monophosphate Kit, Adenosine Monophosphate Colorimetric Assay, AMP Colorimetric Assay, Adenosine Monophosphate, AMP Colorimetric Assay Kit, AMP Assay, Adenosine Monophosphate Colorimetric Kit, AMP

Tag Line : To detect AMP in biological samples

Summary : ’s AMP Colorimetric Assay Kit provides a convenient method to detect AMP in biological samples. In this assay, AMP is converted to pyruvate in the presence of Pyrophosphate and Phosphoenolpyruvate. This is followed by a set of enzymatic reactions to generate a colored product with a strong absorbance at 570 nm. The absorbance is proportional to the amount of AMP present in samples. The kit is rapid, sensitive, easy to use and high-throughput adaptable. It can measure AMP level lower than 10 µM in various sample types.

Detection Method : Absorbance, OD 570 nm

Sample Type : Adherent or suspension Cells: e.g. HeLa, HEK239, Jurkat cells, Tissue lysates: e.g. Liver, Kidney, etc.

Species Reactivity : All

Applications : Study the regulation of AMPK by AMP, Mechanistic study of key cellular processes such as Ca2+ signaling, glucose uptake, lipid uptake, etc., Measurement of AMP level in various cell or tissue types

Features and Benefits :