Anti-AKT (PH domain) Rabbit Monoclonal Antibody (RM316) | A1835

The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-? tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.

A1835 | Anti-AKT (PH domain) Rabbit Monoclonal Antibody (RM316) DataSheet

Antibody Target: AKT

Target Alternative Name:

Tag Line: Affinity purified Rabbit monoclonal to AKT

Category: Primary antibody

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human

Immunogen Sequence: A peptide corresponding to the PH domain of human AKT

Accession #: P31749

Gene ID: 207

Appearance: Liquid

Form: Liquid

Concentration:

Formulation: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Purification: Protein A affinity purified from an animal origin–free culture supernatant

Application: IHC: 1:200-1:500, Western Blot: 1:5000-1:10000

Positive Control:

Application And Usages: IHC: 1:200-1:500, Western Blot: 1:5000-1:10000

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: True

Immunocytochemistry Verified: True

Immunofluorescence Verified: False

Immunoprecipitation Verified: False

FACS Verified: False

ELISA Verified: False

ChIP Verified: False

Dot Blot Verified: False

Flow Cytometry Verified: False