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Anti-AKT (PH domain) Rabbit Monoclonal Antibody (RM316) | A1835
- SKU:
- 26-A1835-50-GEN
- Availability:
- Usually shipped in 5 working days
Description
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 and Akt 3, which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. They have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-? tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
A1835 | Anti-AKT (PH domain) Rabbit Monoclonal Antibody (RM316) DataSheet
Antibody Target: AKT
Target Alternative Name:
Tag Line: Affinity purified Rabbit monoclonal to AKT
Category: Primary antibody
Host: Rabbit
Isotype: Rabbit IgG
Species Reactivities: Human
Immunogen Sequence: A peptide corresponding to the PH domain of human AKT
Accession #: P31749
Gene ID: 207
Appearance: Liquid
Form: Liquid
Concentration:
Formulation: 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Purification: Protein A affinity purified from an animal originfree culture supernatant
Application: IHC: 1:200-1:500, Western Blot: 1:5000-1:10000
Positive Control:
Application And Usages: IHC: 1:200-1:500, Western Blot: 1:5000-1:10000
Country of Animal Origin: USA
Country of Manufacture: USA
Usage: For Research Use Only! Not to be used in humans.
Handling: The antibody solution should be gently mixed before use.
Western Blot Verified: True
Immunocytochemistry Verified: True
Immunofluorescence Verified: False
Immunoprecipitation Verified: False
FACS Verified: False
ELISA Verified: False
ChIP Verified: False
Dot Blot Verified: False
Flow Cytometry Verified: False
Additional Information
Size: |
50 uL |
Storage Condition: |
-20°C |
Shipping Condition: |
Gel Pack |
Shelf Life: |
12 months |