Anti-Diglycyl-Lysine Antibody (Clone# GX41) | A2226

Ubiquitination is one of the most important post-translational modifications that determine the functional activity and stability of the protein. It plays a significant role in multiple biological processes such as cell proliferation, differentiation, regulation of cell cycle, gene transcription, and apoptosis. Changes in the ubiquitination of proteins are linked to diseases such as cancer, inflammation, neurodegenerative, and cardiovascular diseases. Thus, it could be a potential target for drug development. Protein ubiquitination can be identified by mutational experiments and tandem mass spectrometry (MS/MS). However, a new approach relies on an antibody-based detection wherein the antibody recognizes diglycyl-lysine peptide generated as a result of the digestion of protein sample by trypsin. ’s Anti-Diglycyl-Lysine Antibody (Clone# GX41) can help identify the diglycyl lysine sequences that are present on the ?-amine of lysine as an adduct with no reactivity to unmodified lysine.

A2226 | Anti-Diglycyl-Lysine Antibody (Clone# GX41) DataSheet

Antibody Target: Diglycyl-Lysine

Target Alternative Name: Diglycyl-Lysine, Gly-Gly Lycine

Tag Line: Mouse monoclonal antibody to detect peptides with diglycyl modified lysine in human, rat, mouse, chicken, cow, horse, and yeast

Category: Primary antibody

Host: Mouse

Isotype: IgG

Species Reactivities: Tested in Rat. Reactivity expected in Human, Mouse, Chicken, Bovine, Equine, Yeast

Immunogen Sequence: Linear peptide corresponding to Diglycyl Lysine

Accession #:

Gene ID:

Appearance: Lyophilized powder

Form: Lyophilized

Concentration:

Formulation: In 0.2 M HEPES pH 8.5

Purification: Protein A affinity chromatography

Application: Immunoprecipitation (IP),Western Blotting

Positive Control:

Application And Usages: Western Blot, IP

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: TRUE

Immunocytochemistry Verified: FALSE

Immunofluorescence Verified: FALSE

Immunoprecipitation Verified: FALSE

FACS Verified: FALSE

ELISA Verified: FALSE

ChIP Verified: FALSE

Dot Blot Verified: FALSE

Flow Cytometry Verified: FALSE