ATG7 Antibody (APG7)| F46227-0.08ML

ATG7 Antibody (APG7)| F46227-0.08ML | Gentaur UK, US & Europe Distribution

Background: ATG7 is an E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. [UniProt]

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human, Mouse

Predicted Spacies Reactivity: Rat

Application: WB, IHC, ELISA

Application Details: Western blot: 1:1000, IHC (Paraffin): 1:50-1:100

Application Note: Titration of the ATG7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This ATG7 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 22-51 from the human protein was used as the immunogen for this ATG7 antibody.

Storage: Aliquot the ATG7 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A