ATR Antibody | 3117

ATR Antibody | 3117 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: ATR antibody was raised against a peptide corresponding to 13 amino acids near the center of human ATR.
The immunogen is located within amino acids 220 - 270 of ATR.

Research Area: Neuroscience, Infectious Disease

Tested Application: E, WB, IHC-P, IF

Application: ATR antibody can be used for detection of ATR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least three isoforms of ATR are known to exist; this antibody will detect all three isoforms.

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 3: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 36, 40, 60 kDa
Observed: 45 kDa

Validation: N/A

Isoform: N/A

Purification: ATR Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: ATR Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: ATR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: ATR Antibody: ATR, GAPO, TEM8, ATR, Anthrax toxin receptor 1, Tumor endothelial marker 8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: ATR Antibody: The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8) . This protein, which exists in three isoforms (36, 40, and 60 kDa) , is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF) . This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.