AXIN1 Antibody| F45423-0.08ML/F45423-0.4ML/R34056-100UG

AXIN1 Antibody| F45423-0.08ML/F45423-0.4ML/R34056-100UG | Gentaur UK, US & Europe Distribution

Background: AXIN1 is a component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development. [UniProt]

Format: Antigen affinity purified

Clone: N/A

Host Animal: Rabbit

Clonality: Polyclonal (rabbit origin)

Isotype: Rabbit Ig

Species Reactivity: Human

Predicted Spacies Reactivity: Mouse, Rat

Application: WB, ELISA

Application Details: Western blot: 1:1000

Application Note: Titration of the AXIN1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Status: Available

Buffer: N/A

Limitation: This AXIN1 antibody is available for research use only.

Purity: Antigen affinity

Immunogen: A portion of amino acids 330-359 from the human protein was used as the immunogen for this AXIN1 antibody.

Storage: Aliquot the AXIN1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Localization: N/A