Caspase-9 Antibody | 3136

Caspase family of cysteine proteases plays a key role in apoptosis. Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase-9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17-25 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.

3136 | Caspase-9 Antibody DataSheet

Antibody Target: Caspase-9

Target Alternative Name: CASP-9 ,EC 3.4.22.62 ,ICE-like apoptotic protease 6 , ICE-LAP6,Apoptotic protease Mch-6 ,Apoptotic protease-activating factor 3 , Apaf-3

Tag Line:

Category: Caspase

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human, mouse, rat

Immunogen Sequence: Synthetic peptide surrounding amino acid 290 of mouse caspase-9

Accession #: BAA86895

Gene ID: 12371

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.2 mg/ml

Formulation: In phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved immediate fragments and the 17-25 kDa fully cleaved caspase-9.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified