Cattle Bcl2 (B-Cell Leukemia/Lymphoma 2) ELISA Kit | ELK6902

Cattle Bcl2 (B-Cell Leukemia/Lymphoma 2) ELISA Kit | ELK6902 | Gentaur UK, US & Europe Distribution

Alternative Names: B-Cell CLL/Lymphoma 2

Species: Cattle

Assay Type: Sandwich

Sensitivity: 0.55 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Apoptosis

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Cell Leukemia/Lymphoma 2 (Bcl2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell Leukemia/Lymphoma 2 (Bcl2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell Leukemia/Lymphoma 2 (Bcl2) in the samples is then determined by comparing the OD of the samples to the standard curve.