Cattle CSN3 (Casein Kappa) ELISA Kit | ELK8007

Cattle CSN3 (Casein Kappa) ELISA Kit | ELK8007 | Gentaur UK, US & Europe Distribution

Alternative Names: CSN10; CSNk; KCA; CASK

Species: Cattle

Assay Type: Sandwich

Sensitivity: 4.3 ng/mL

Standard: 600 ng/mL

Detection Range: 9.38-600 ng/mL

Sample Type: milk

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kappa (CSN3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Casein Kappa (CSN3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kappa (CSN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Casein Kappa (CSN3) in the samples is then determined by comparing the OD of the samples to the standard curve.