Cattle GDH (Glutamate Dehydrogenase 1) ELISA Kit | ELK5953

Cattle GDH (Glutamate Dehydrogenase 1) ELISA Kit | ELK5953 | Gentaur UK, US & Europe Distribution

Alternative Names: GLUD1; Glutamate dehydrogenase 1, mitochondrial

Species: Cattle

Assay Type: Sandwich

Sensitivity: 3.1 ng/mL

Standard: 500 ng/mL

Detection Range: 7.82-500 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Endocrinology;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Dehydrogenase 1 (GDH) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutamate Dehydrogenase 1 (GDH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Dehydrogenase 1 (GDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Dehydrogenase 1 (GDH) in the samples is then determined by comparing the OD of the samples to the standard curve.