CD49b Antibody [DX5] (Biotin) | 98-960

CD49b Antibody [DX5] (Biotin) | 98-960 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: NK cells isolated from C57BL/6 mice

Research Area: Immunology

Tested Application: Flow

Application: CD49b Antibody [DX5] for use in flow cytometry and immunohistochemistry assays.

Specificiy: CD49b

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [DX5]

Isotype: IgM

Conjugate: Biotin

Physical State: Liquid

Buffer: Supplied in PBS/NaN3

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: alpha2 integrin, VLA-2alpha, very late antigen-2, pan NK

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and alpha2 integrin. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR) . Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes. NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity.