CNTF Antibody (biotin) | XP-5114Bt

CNTF Antibody (biotin) | XP-5114Bt | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Rat

Homology: N/A

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat CNTF (rat CNTF) .

Research Area: Chemokines & Cytokines, Antibody Pairs

Tested Application: E, WB

Application: ELISA:
Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat CNTF (XP-5114) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.
Western Blot:
To detect rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat CNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Anti-rat CNTF specific antibody was purified by affinity chromatography and then biotinylated.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Biotin

Physical State: Lyophilized

Buffer: N/A

Concentration: N/A

Storage Condition: CNTF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.

Alternate Name: Ciliary neurotrophic factorCNTF

User Note: Centrifuge vial prior to opening.

BACKGROUND: Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury.