CRY1 (Human) ELISA Kit - E4994

E4994 | CRY1 (Human) ELISA Kit DataSheet

Cryptochrome 1 (CRY1) is a photolyase-like-blue light receptor that belongs to the class of flavoproteins. It was first identified in Arabidopsis and later discovered in other lineages. In Arabidopsis, CRY1 mediates inhibition of hypocotyl elongation in response to blue light and photo periodically control floral initiation. In Drosophila, CRY1 binds to TIM in a light-dependent manner and mediates its degradation. In mammals, CRY1 heterodimerizes with PERIOD proteins, enters the nucleus, and suppresses CLOCK-BMAL1 transcriptional activity in a light-independent manner, thereby forming a negative feedback loop of the mammalian circadian clock. Additional functions of CRY1 include the regulation of glucose homeostasis and proinflammatory cytokine production. CRY1 is also known to downregulate the cAMP/PKA signaling pathway and promote cell growth and proliferation. Overexpression of CRY1 is associated with chronic lymphocytic leukemia and colorectal cancers and thus may serve as a promising target for anticancer therapy. ’s CRY1 (Human) ELISA kit quantitatively measures CRY1 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. HRP-Streptavidin is added to the plate, and post-incubation any unattached conjugates are washed off by Wash Buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of CRY1 in the sample or standard.

Alternate Name : DSPD, PHLL1, CRY1, Cryptochrome-1

Tag Line : A Sandwich ELISA kit for quantitative measurement of CRY1 in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Serum Plasma Tissue lysate Other biological fluids

Species Reactivity : Human

Applications : Sandwich ELISA kit to quantitatively measure CRY1 in human serum, plasma, tissue lysate, and other biological fluids

Features and Benefits : Detection range: 0.313 – 20 ng/ml This Sandwich ELISA is highly sensitive and highly specific for the detection of CRY1 in human samples. There is no significant cross-reactivity or interference between CRY1 and analogues Sensitivity: 0.188 ng/ml Recovery range: 85 - 105% for normal human serum and plasma samples Assay Precision: Intra-Assay CV and Inter-Assay CV < 10%