General THFA (Tetrahydrofolic Acid) ELISA Kit | ELK8034

General THFA (Tetrahydrofolic Acid) ELISA Kit | ELK8034 | Gentaur UK, US & Europe Distribution

Alternative Names: H4FA; THF; Tetrahydrofolate

Species: General

Assay Type: Competitive Inhibition

Sensitivity: 0.57 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Tetrahydrofolic Acid (THFA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tetrahydrofolic Acid (THFA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetrahydrofolic Acid (THFA) in the samples is then determined by comparing the OD of the samples to the standard curve.