GFAP (Human) ELISA Kit - E5049

E5049 | GFAP (Human) ELISA Kit DataSheet

GFAP, Glial Fibrillary Acidic Protein is an intermediate filament protein which belongs to the class-III intermediate filament family. GFAP is expressed in numerous cell types of the central nervous system, ependymal cells and phosphorylated by PKN1. GFAP acts as a cell-specific marker during the development of the central nervous system and distinguishes astrocytes from other glial cells. It is involved in many important CNS processes, including cell communication and the functioning of the blood brain barrier. GFAP and its breakdown products are rapidly released into biofluids during traumatic brain and spinal cord injuries and stroke, making them a strong candidate bio-markers for such neurological disorders. ’s GFAP (Human) ELISA Kit is used for the quantitative detection of GFAP in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of GFAP in the sample.

Alternate Name : GFAP ELISA Kit; FLJ45472 ELISA Kit; glial fibrillary acidic protein ELISA Kit; ALXDRD ELISA Kit

Tag Line : A highly sensitive ELISA kit for quantitative measurement of GFAP

Summary :

Detection Method : Absorbance is measured at 450 nm

Sample Type : Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity : Human

Applications : The ELISA kit is used for the quantitative detection of GFAP in serum, plasma, tissue homogenates and other biological fluids

Features and Benefits : Detection range: 0.313-20 ng/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Sensitivity: 0.188 ng/ml Recovery Rate: 85-104% for Serum, 87-103% for EDTA plasma and 87-99% for Heparin plasma Highly sensitive and specific