Glutamine Synthetase Inhibitor Screening Kit (Fluorometric) - K2059

K2059 | Glutamine Synthetase Inhibitor Screening Kit (Fluorometric) DataSheet

Glutamine Synthetase (GS; EC 6.3.1.2) is a key enzyme that regulates nitrogen metabolism by catalyzing the formation of glutamine from glutamate and ammonia. It is ubiquitously expressed in humans and is linked to several neurological disorders including Alzheimer’s disease, schizophrenia, epilepsy etc. Inhibition of GS in animal models have shown significant therapeutic benefits in hepatic encephalopathy, amyotrophic lateral sclerosis, inflammatory liver failure, cancer and other human diseases. Additionally, GS is highly expressed in bacteria and is essential for its growth. Inhibition of Mycobacterium tuberculosis GS has been used as a therapeutic strategy against Tuberculosis. ’s Glutamine Synthetase Inhibitor Screening Kit is a plate-based fluorometric assay for the screening human GS inhibitors. In this kit, the activity of GS is monitored by the formation of a fluorescent product formed via an enzymatic reaction and is detected at Ex/Em = 535/587 nm. But in the presence of GS inhibitors, the GS enzyme loses its activity, which results in the decrease of fluorescence intensity. Methionine Sulfoximine (MSO), a well-characterized inhibitor of prokaryotic and eukaryotic GSs with different Ki values is used as the positive control. The assay is simple and can used to identify and characterize human GS inhibitors.

Alternate Name : Glutamine Synthetase Inhibitor Screening,Glutamine Synthetase Inhibitor Kit (Fluorometric),GS Inhibitor Screening,GS Inhibitor Screening Kit,GS Inhibitor Screening Kit (Fluorometric),Glutamine Synthetase Inhibitor Screening Kit,Glutamine Synthetase Inhibitor Kit (Fluorometric),GS Inhibitor Kit (Fluorometric)

Tag Line : For the screening human Glutamine Synthetase inhibitors

Summary : ’s Glutamine Synthetase Inhibitor Screening Kit is a plate-based fluorometric assay for the screening human GS inhibitors. In this kit, the activity of GS is monitored by the formation of a fluorescent product formed via an enzymatic reaction and is detected at Ex/Em = 535/587 nm. But in the presence of GS inhibitors, the GS enzyme loses its activity, which results in the decrease of fluorescence intensity.

Detection Method : Fluorescence Ex/Em = 535/587 nm

Sample Type :

Species Reactivity : Human

Applications : Screening of potential inhibitors of Human Glutamine Synthetase.

Features and Benefits : A plate-based fluorometric assay for the screening human GS inhibitors.,The assay is simple and easy to use.