Glycine N-methyltransferase, Human Recombinant | P1235

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1235 | Glycine N-methyltransferase Human Recombinant DataSheet

Biomolecule/Target: GNMT

Synonyms: GNMT

Alternates names: Apolipoprotein E4, APOE, apolipoprotein, apolipoproteins

Taglines: Regulate tissue concentration of AdoMet and metabolism of methionine.

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #:

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per/g

Activity (Specifications/test method): Measured by its ability to transfer 1.0 nmole of methyl group per minute at 37C.

Biological activity: N/A

Results: >100 nmol/min/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 34.4 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Reconstitution Instructions: Reconstitute in dHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A