GPX | Glutathione peroxidase (chloroplastic) | AS04 055

GPX | Glutathione peroxidase (chloroplastic) | AS04 055 | Gentaur UK, US & Europe Distribution

Immunogen: thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala of Arabidopsis thaliana GPX7 accession At4g31870 has been used for antibody production

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: ELISA (ELISA), Western blot (WB)

Related Products: AS06 181 | Anti-glutatione reductase rabbit antibodiesPlant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)

Molecular weight: 25 | 20 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: This antibody is recognizing Arabidopsis thaliana GPX (two chloroplastic isoforms) . Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

Background: GPX belongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS) . Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.

Reconstitution: For reconstitution add 100 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G31870

Category: Oxidative stress

Research Area: Plant Biology