Human Period circadian protein homolog 1 (PER1) ELISA Kit | AE28006HU

Human Period circadian protein homolog 1 (PER1) ELISA Kit | AE28006HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: PER1

Alternative Name: MGC88021; PER; RIGUI; hPER; Period; drosophila; homolog of|circadian pacemaker protein RIGUI|period 1

Application: ELISA

Range: 0.312-20 ng/mL

Sensitivity: 0.122 ng/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤9.2%

Recovery: 1, 01

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PER1 in samples. An antibody specific for PER1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPER1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PER1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PER1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: PER1 is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. Circadian expression in the suprachiasmatic nucleus continues in constant darkness, and a shift in the light/dark cycle evokes a proportional shift of gene expression in the suprachiasmatic nucleus. The specific function of this gene is not yet known. Alternative splicing has been observed in this gene; however, these variants have not been fully described.Widely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .