Human SCNN1b (Amiloride Sensitive Sodium Channel Subunit Beta) ELISA Kit | ELK4240

Human SCNN1b (Amiloride Sensitive Sodium Channel Subunit Beta) ELISA Kit | ELK4240 | Gentaur UK, US & Europe Distribution

Alternative Names: ENaCb; ENaCbeta; SCNEB; Liddle Syndrome; Sodium Channel, Nonvoltage-Gated 1, Beta; Epithelial Na (+) channel subunit beta; Nonvoltage-gated sodium channel 1 subunit beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.107 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amiloride Sensitive Sodium Channel Subunit Beta (SCNN1b) in the samples is then determined by comparing the OD of the samples to the standard curve.