Human Semaphorin-3B (SEMA3B) ELISA Kit | AE20190HU

Human Semaphorin-3B (SEMA3B) ELISA Kit | AE20190HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SEMA3B

Alternative Name: FLJ34863; LUCA-1; SEMA5; SEMAA; SemA; semaV; semaphorin 3B|semaphorin A|semaphorin V

Application: ELISA

Range: 0.156-10 ng/mL

Sensitivity: 0.056 ng/mL

Intra-Assay: ≤5.9%

Inter-Assay: ≤9.8%

Recovery: 1, 11

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SEMA3B in samples. An antibody specific for SEMA3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySEMA3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The semaphorin/collapsin family of molecules plays a critical role in the guidance of growth cones during neuronal development. The secreted protein encoded by this gene family member is important in axonal guidance and has been shown to act as a tumor suppressor by inducing apoptosis. The 2 genes have widespread but distinct patterns of expression in nonneural tissues, and have different patterns of expression in lung cancer. Human semaphorin A (V) has 86% amino acid homology with murine semaphorin A, whereas semaphorin IV is more closely related to murine semaphorin E, with 50% homology.In particular, overlapping homozygous deletions in lung cancers have been identified in region 3p21.3; more than 90% of small cell lung cancers exhibit LOH at this site.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .