Isotype Control

Isotype Control

Isotype control is a critical component in immunological research, particularly in experiments involving antibodies. It refers to an antibody of the same isotype (class and subclass) as the primary antibody being used in an experiment but directed against an antigen that is not present in the sample or tissue under investigation.

Isotype Control Antibodies: Overview & Usage

Here's why isotype controls are important and how they are used:

Control for Non-specific Binding: Isotype controls help researchers distinguish specific binding of the primary antibody to its target antigen from non-specific binding. Non-specific binding can occur due to interactions between the primary antibody and unrelated proteins or structures in the sample. By using an isotype control antibody that should not bind to any antigens in the sample, researchers can identify and account for non-specific background signal.

Control for Fc Receptor Binding: Isotype controls are also useful for assessing binding to Fc receptors on cells, which can lead to false-positive results if not properly controlled. By using an isotype control antibody that has the same Fc region as the primary antibody, researchers can evaluate and account for any binding of the primary antibody to Fc receptors on cells.

Standardization and Reproducibility: Isotype controls help standardize experimental procedures and ensure reproducibility across experiments by providing a consistent control for background signal. Including an isotype control alongside each experimental sample allows researchers to compare signal intensity and specificity between samples and experiments.

Negative Control: Isotype controls serve as negative controls in immunostaining experiments, where they are used to determine the level of background staining in the absence of specific antigen-antibody interactions. This information is crucial for accurately interpreting the staining patterns observed in experimental samples.

  • Product
  • Qty in Cart
  • Quantity
  • Price
  • Subtotal
  • Normal Chicken Serum

    Normal Chicken Serum | RS-30-100ML

    100

    NULL243.00
    Normal Chicken Serum | RS-30-100ML | Gentaur Species Reactivity: Chicken Format: Serum Product Type: Isotype Control Antibody clonality: Polyclonal
    100-RS-30-100ML
    NULL243.00
    Qty in Cart: 0
    Quantity:
    Price:
    NULL243.00
    Subtotal:
  • Rabbit IgG Enriched

    Rabbit IgG Enriched | RA-15G

    100

    NULL145.00
    Rabbit IgG Enriched | RA-15G | Gentaur Species Reactivity: Rabbit Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal
    100-RA-15G
    NULL145.00
    Qty in Cart: 0
    Quantity:
    Price:
    NULL145.00
    Subtotal:
  • Mouse IgG Enriched

    Mouse IgG Enriched | RA-90G

    100

    NULL259.00
    Mouse IgG Enriched | RA-90G | Gentaur Species Reactivity: Mouse Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal
    100-RA-90G
    NULL259.00
    Qty in Cart: 0
    Quantity:
    Price:
    NULL259.00
    Subtotal:
  • Chicken IgY Enriched

    Chicken IgY Enriched | RA-30Y

    100

    NULL243.00
    Chicken IgY Enriched | RA-30Y | Gentaur Species Reactivity: Chicken Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal
    100-RA-30Y
    NULL243.00
    Qty in Cart: 0
    Quantity:
    Price:
    NULL243.00
    Subtotal:
  • Total: items /

Adding your products to cart