Leptin Antibody | 5368

Leptin is a recently identified protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. The expression of leptin mRNA has been shown to be restricted to adipose tissue. Leptin plays an important role in reproduction, immunological response and neuroendocrine signaling.

5368 | Leptin Antibody DataSheet

Antibody Target: Leptin

Target Alternative Name: LEP; OB; OBS; Obese Protein, Leptin

Tag Line:

Category: Lepitn

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Rat

Immunogen Sequence: E. coli-expressed recombinant rat Leptin

Accession #: BAA08529

Gene ID: 25608

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: 100 µg (0.5 mg/ml) affinity purified rabbit anti-rat Leptin polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blot analysis (0.5-2 µg/ml). However, the optimal conditions should be determined individually. Recombinant rat Leptin (Cat.# 4368-02) can be used as a positive control. Other applications have not been tested.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified