Mouse Pyrroline-5-carboxylate reductase 2 (PYCR2) ELISA Kit | KTE70594

Mouse Pyrroline-5-carboxylate reductase 2 (PYCR2) ELISA Kit | KTE70594 | Gentaur UK, US & Europe Distribution

Application: This Mouse Pyrroline-5-carboxylate reductase 2 (PYCR2) ELISA Kit employs a two-site sandwich ELISA to quantitate PYCR2 in samples. An antibody specific for PYCR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPYCR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYCR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYCR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Mouse Pyrroline-5-carboxylate reductase 2 microplate
• Mouse Pyrroline-5-carboxylate reductase 2 standard
• Mouse Pyrroline-5-carboxylate reductase 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Mouse Pyrroline-5-carboxylate reductase 2 (PYCR2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PYCR2. No significant cross-reactivity or interference between Mouse PYCR2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PYCR2, Belongs to the pyrroline-5-carboxylate reductase family. Pyrroline-5-carboxylate reductase (P5CR) is the enzyme that catalyzes the terminal step in the biosynthesis of proline from glutamate, the NAD (P) dependent oxidation of 1-pyrroline-5-carboxylate into proline. Pyrroline-5-carboxylate reductase, which converts pyrroline-5-carboxylate to proline, has been identified in human erythrocytes. The level of pyrroline-5-carboxylate reductase activity in these cells is comparable to the activity levels of major erythrocyte enzymes. The physiologic function of the enzyme in erythrocytes cannot be related to its function in other tissues, i.e., producing proline for protein synthesis.

Alternative Names: PYCR2; RP4-559A3.4; FLJ54750; P5CR2; pyrroline 5-carboxylate reductase isoform

Search name: PYCR2; RP4-559A3.4; FLJ54750; P5CR2; pyrroline 5-carboxylate reductase isoform

Tag: PYCR2