NANOG Antibody | 45-931

NANOG Antibody | 45-931 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Dog, Pig

Immunogen: The immunogen for this antibody is: C-QNQRMKSKRWQKNN

Research Area: Cancer

Tested Application: E, WB, IF, IHC, EIA

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:This antibody was successfully used in: Vassena et al, Hum Mol Genet. 2012 Aug 1;21 (15) :3366-73. PMID: 22547223, Sancho-Martinez et al, Nat Commun. 2016 Feb 22;7:10743. PMID: 26899176, and Montserrat et al, Cell Transplant. 2012;21 (5) :815-25. PMID: 21944493.Immunofluorescence: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green) .
This antibody was successfully used in IF on Human: Estibaliz Arellano-Viera et al. (2019) https://doi.org/10.1016/j.scr.2019.101410. This anyibody was successfully used in ICC on Human: Stem Cell Research (2019) , Enzyme immunoassay: This antibody is reported to work in sandwich-type ELISA both as a capturer and as a reporter in combination with EB07693. This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.

Specificiy: N/A

Positive Control 1: Cat. No. 1316 - Human Ovary Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NANOG, Nanog homeobox, HGNC:20857, homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

User Note: N/A

BACKGROUND: N/A