Nucleophosmin Antibody | 45-099

Nucleophosmin Antibody | 45-099 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-QEAIQDLWQWRKSL

Research Area: Homeostasis

Tested Application: E, WB, IHC, IF

Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_032748.1) . This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Testis. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10µg/ml.

Specificiy: N/A

Positive Control 1: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: NPM1, NPM, B23, nucleophosmin (nucleolar phosphoprotein B23, numatrin) , numatrin, nucleolar phosphoprotein B23, nucleophosmin/nucleoplasmin family, member 1, MGC104254, nucleophosmin 1

User Note: N/A

BACKGROUND: N/A