p53 Antibody | 3036R

p53, a 53 kDa protein, binds to a DNA consensus sequence, the p53 response element, and regulates normal cell cycle events by activating transcription of genes involved either in progression thro?gh the cell cycle, or causing arrest in G1 when the genome is damaged. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the concentration in normal cells, principally due to the increased half-life (4 hrs) compared to that of wild-type (20 min). p53 localizes in the nucleus, but is detectable at the plasma membrane during mitosis and certain mutations also modulate cytoplasmic/nuclear distribution. p53 downregulates Bcl-2 expression and upregulates Bax expression, but may not always be necessary for apoptosis.

3036R | p53 Antibody DataSheet

Antibody Target: p53

Target Alternative Name: TP53, TRP53, P53, LFS1, Tumor suppressor p53, Phosphoprotein p53

Tag Line:

Category: p53

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human, mouse, rat, dog, rabbit

Immunogen Sequence: Synthetic peptide corresponding to residue surrounding amino acid 375 of human p53

Accession #: EAW90140

Gene ID: 7157

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blot analysis (0.5-4 µg/ml). It detects p53 in samples of human, mouse and rat origins. Other applications have not been determined.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified: Not Verified

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified: Not Verified

Flow Cytometry Verified: Not Verified