p53R2 Antibody | 2383

p53R2 Antibody | 2383 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: p53R2 antibody was raised against a 16 amino acid peptide near the amino terminus of human p53R2.
The immunogen is located within the first 50 amino acids of p53R2.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: p53R2 antibody can be used for detection of p53R2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least six isoforms of p53R2 are known to exist; this antibody will detect all but the largest isoform.

Positive Control 1: Cat. No. 1202 - A431 Cell Lysate

Positive Control 2: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 3: Cat. No. 1302 - Human Lung Tissue Lysate

Positive Control 4: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 39 kDa
Observed: 43 kDa

Validation: N/A

Isoform: N/A

Purification: p53R2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: p53R2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: p53R2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: p53R2 Antibody: P53R2, MTDPS8A, MTDPS8B, P53R2, Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53R2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: p53R2 Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2, Chk2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase. p53R2 is induced by the reagents, ultraviolet and gamma-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression. p53R2 is an important target of p53 for tumor suppression.