PADI4 (Human) ELISA Kit - E4969

E4969 | PADI4 (Human) ELISA Kit DataSheet

Protein-arginine deiminase type-4 (PADI4) is an enzyme that belongs to the family of peptidyl arginine deiminases. PADI4 is expressed in immune cells, uterus, bone marrow, and brain. Similar to PADI2, PADI4 also catalyzes the conversion of arginine residues of proteins to citrulline, in the presence of calcium ions. PADI4 also is known to catalyze the citrullination of histones and in turn, regulate transcription and organization of the chromatin. PADI4 mediated histone citrullination plays an important role in DNA damage. Upregulation of PADI4 levels are observed in multiple tumors such as hepatocellular carcinoma, uterine, ovarian colorectal and lung adenocarcinomas. ’s PADI4 (Human) ELISA kit is based on the Sandwich principle to quantitatively measure the concentration of PADI4 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is directly proportional to the concentration of PADI4 in the sample or standard.

Alternate Name : PAD, PAD4, PDI4, PDI5, PADI5, PADI4

Tag Line : A Sandwich ELISA kit for quantitative measurement of Peptidyl Arginine Deiminase Type IV (PADI4) in human serum, plasma, tissue lysates, and other biological fluids

Summary :

Detection Method : Absorbance (450 nm)

Sample Type : Plasma Tissue lysate Serum Other biological fluids

Species Reactivity : Human

Applications : Sandwich ELISA kit to quantitatively measure PADI4 in human serum, plasma, tissue lysate, and other biological fluids

Features and Benefits : Assay Precision: Intra-Assay CV < 8% and Inter-Assay CV < 10% Sensitivity: 0.113 ng/ml This Sandwich ELISA is highly sensitive and highly specific for the detection of PADI4 in human samples. There is no significant cross-reactivity or interference between PADI4 and analogues Recovery range: 85 - 105% for normal human serum and plasma samples Detection range: 0.188 – 12 ng/ml