Description
Parvalbumin Antibody | 46-135 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human, Rat
Homology: N/A
Immunogen: The immunogen for this antibody is: C-GVDEFSTLVAES
Research Area: Cancer
Tested Application: E, WB, Flow, IHC
Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 14-15kDa band observed in Human (Cerebellum) Brain lysates (calculated MW of 12.1kDa according to NP_002845.1) . Primary incubation 1 hour at room temperature. Recommended concentration: 0.1-0.3ug/ml. Approx 10-12kDa band also observed in Rat Skeletal Muscle and in Rat Heart cells transfected with human PVALB, but not in untransfected Rat Heart cells. Data kindly provided by Dr Wang Wang, University of Michigan.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) . Recommended concentration: 2.5ug/ml.Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.
Specificiy: Reported variants represent identical protein: NP_002845.1, NP_001302461.1
Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: parvalbumin, HGNC:9704, PVALB, D22S749, parvalbumin alpha, MGC116759
User Note: N/A
BACKGROUND: N/A