Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening Kit - K2012

K2012 | Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening Kit DataSheet

Phosphoenolpyruvate Carboxykinase (PEPCK, EC 4.1.1.32) is an enzyme, which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, PEPCK1 or PCK1) and mitochondrial form (PEPCK-M). PEPCK1 is a rate-controlling step in gluconeogenesis and a key gluconeogenic enzyme in liver and kidney. Recent studies have shown that overexpressing of PEPCK1 in liver leads to excessive glucose production and type II diabetic phenotype in mice. Therefore, identifying and screening PEPCK1 inhibitors is a useful strategy for the treatment of type II diabetes and obesity. In ’s Human Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening Kit, PEPCK1 is coupled with a set of enzymes that covert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn reacts with a probe thereby generating a colorimetric signal (OD 570 nm). In the presence of PEPCK1 Inhibitor, the reaction is impeded. A PEPCK1 Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is high-throughput adaptable and can be completed in less than 1 hr.

Alternate Name : PEPCK Inhibitor Kit, PEPCK Inhibitor Screening Assay Kit, Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening, PEPCK Inhibitor Screening Assay, PEPCK, PEPCK Inhibitor Screening, Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening, PEPCK Inhibitor Assay, PEPCK Inhibitor Screening Kit, Phosphoenolpyruvate Carboxykinase 1 Inhibitor Kit, PEPCK1, Phosphoenolpyruvate Carboxykinase Inhibitor Screening Kit

Tag Line : for screening/characterizing/studying potential inhibitors of Human PEPCK1

Summary : Phosphoenolpyruvate Carboxykinase (PEPCK, EC 4.1.1.32) is an enzyme, which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, PEPCK1 or PCK1) and mitochondrial form (PEPCK-M). PEPCK1 is a rate-controlling step in gluconeogenesis and a key gluconeogenic enzyme in liver and kidney. Recent studies have shown that overexpressing of PEPCK1 in liver leads to excessive glucose production and type II diabetic phenotype in mice. Therefore, identifying and screening PEPCK1 inhibitors is a useful strategy for the treatment of type II diabetes and obesity. In ’s Human Phosphoenolpyruvate Carboxykinase 1 Inhibitor Screening Kit, PEPCK1 is coupled with a set of enzymes that covert PEP and carbonate into a series of intermediates and hydrogen peroxide, which in turn reacts with a probe thereby generating a colorimetric signal (OD 570 nm). In the presence of PEPCK1 Inhibitor, the reaction is impeded. A PEPCK1 Inhibitor Control is included to compare the efficacy of the sample inhibitors. The assay is high-throughput adaptable and can be completed in less than 1 hr.

Detection Method : Absorbance, OD 570 nm

Sample Type : Test compounds

Species Reactivity : Mammalian

Applications : Screening/characterizing/studying potential inhibitors of Human PEPCK1

Features and Benefits : High-throughput adaptable, Simple, Quick