PIERCE1 Antibody | 6721

PIERCE1 Antibody | 6721 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: PIERCE1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human PIERCE1.
The immunogen is located within the first 50 amino acids of PIERCE1.

Research Area: Apoptosis

Tested Application: E, WB, ICC

Application: PIERCE1 antibody can be used for detection of PIERCE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: PIERCE1 antibody is predicted to not cross-react with other UPF0691 family members.

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 17-208 - A-20 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: PIERCE1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PIERCE1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: PIERCE1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: PIERCE1 Antibody: PIERCE1, RbEST47, UPF0691 protein C9orf116, p53-induced expression in RB-null cells protein 1, Pierce1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: PIERCE1 Antibody: PIERCE1, also known as C9orf116, is an important p53 target gene contributing to normal DNA damage response and may play crucial roles in maintaining genomic integrity against genotoxic stresses, including UVC irradiation. PIERCE1 exists as two alternatively spliced isoforms and is predominantly expressed in human small cell lung cancer. PIERCE1 knockdown induces down-regulation of proapoptotic genes including Ei24, Apaf1 and PTEN, suggesting that PIERCE1 is a proapoptotic gene. It is helpful for in-depth understanding of p53 pathways and the design of new cancer therapies.