Description
PNGase F is an amidohydrolase, which cleaves the β-aspartylglucosamine bond between the innermost GlcNAc of glycans and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins and glycopeptides. The cleavage results in a deaminated protein or peptide and free glycans. PNGase F is widely used in the de-glycosylation of glycoproteins to study glycans and identify glycosylation sites.
P1598 | PNGase F, Recombinant DataSheet
Biomolecule/Target :
Synonyms: N-glycosidase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F protein Peptide N-Glycosidase F protein PNGase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F Peptide N-Glycosidase F Recombinant PNGase F protein
Alternates names: N-glycosidase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F protein Peptide N-Glycosidase F protein PNGase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F Peptide N-Glycosidase F Recombinant PNGase F protein
Taglines: An amidohydrolase to study glycans
NCBI Gene ID #:
NCBI Gene Symbol:
Gene Source: Human
Accession #: Q9XBM8 (1-314 aa)
Recombinant: TRUE
Source: E.coli
Purity by SDS-PAGE: ≥ 95% by SDS-PAGE
Assay:
Purity:
Assay #2:
Endotoxin Level:
Activity (Specifications/test method): ≥ 10 U/mg
Biological activity: Biological activity is performed using ’s pNGase F Deglycosylation Kit ( Cat # K2061-100).
Results:
Binding Capacity:
Unit Definition: One unit of pNGase F is defined as the amount of enzyme that catalyzes the deglycosylation of 1 umole of denatured ribonuclease B (RNase B) in one minute when 50% RNase B (20 ug) is deglycosylated for 60 min at 37°C and monitored on an SDS-PAGE under the assay conditions.
Molecular Weight: 37.1 kDa
Concentration: Lot dependent
Appearance: Liquid protein
Physical form description:
Reconstitution Instructions:
Amino acid sequence:
Handling:
Usage: For Research Use Only! Not to be used in humans
Additional Information
Storage Temperature: |
-20°C |
Shipping: |
Gel Pack |
Shelf Life: |
24 months |