PNGase F, Recombinant | P1598

PNGase F is an amidohydrolase, which cleaves the β-aspartylglucosamine bond between the innermost GlcNAc of glycans and asparagine residues of high mannose, hybrid and complex oligosaccharides from N-linked glycoproteins and glycopeptides. The cleavage results in a deaminated protein or peptide and free glycans. PNGase F is widely used in the de-glycosylation of glycoproteins to study glycans and identify glycosylation sites.

P1598 | PNGase F, Recombinant DataSheet

Biomolecule/Target :

Synonyms: N-glycosidase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F protein Peptide N-Glycosidase F protein PNGase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F Peptide N-Glycosidase F Recombinant PNGase F protein

Alternates names: N-glycosidase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F protein Peptide N-Glycosidase F protein PNGase F Peptide-N4-(N-acetyl-β-D-glucosiminyl) asparagine amidase F Peptide N-Glycosidase F Recombinant PNGase F protein

Taglines: An amidohydrolase to study glycans

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: Human

Accession #: Q9XBM8 (1-314 aa)

Recombinant: TRUE

Source: E.coli

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 10 U/mg

Biological activity: Biological activity is performed using ’s pNGase F Deglycosylation Kit ( Cat # K2061-100).

Results:

Binding Capacity:

Unit Definition: One unit of pNGase F is defined as the amount of enzyme that catalyzes the deglycosylation of 1 umole of denatured ribonuclease B (RNase B) in one minute when 50% RNase B (20 ug) is deglycosylated for 60 min at 37°C and monitored on an SDS-PAGE under the assay conditions.

Molecular Weight: 37.1 kDa

Concentration: Lot dependent

Appearance: Liquid protein

Physical form description:

Reconstitution Instructions:

Amino acid sequence:

Handling:

Usage: For Research Use Only! Not to be used in humans