Podocalyxin, mouse recombinant | P1033

PODXL, also known as Podocalyxin, is a heavily glycosylated transmembrane sialoprotein in the CD34 and endoglycan family. This protein involved in the regulation of both adhesion and cell morphology and cancer progression. It has to function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Also, it acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Recombinant mouse PODXL, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1033 | Podocalyxin mouse recombinant DataSheet

Biomolecule/Target: Podxl

Synonyms: AW121214, Ly102, PC, PCLP-1, Pclp1, Podxl

Alternates names: Activin beta-B, Inhibin beta-2

Taglines: N/A

NCBI Gene ID #: 91

NCBI Gene Symbol: ACVR1B

Gene Source: Human

Accession #: P36896

Recombinant: Yes

Source: (BTI-Tn-5B1-4) Hi-5 Insect cells

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is 2.0 ng/ml, corresponding to a specific activity of 5 x 105 units/mg.

Biological activity: Determined by its ability to inhibit the proliferation of mouse MPC-11 cells. The expected ED50 is 2.0 ng/ml, corresponding to a specific activity of 5 x 105 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.6 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 5% Trehalose.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in 10 mM Sodium citrate, pH 3.0 to a concentration of 0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: GLECDGRTNL CCRQQFFIDF RLIGWNDWII APTGYYGNYC EGSCPAYLAG VPGSASSFHT AVVNQYRMRG LNPGTVNSCC IPTKLSTMSM LYFDDEYNIV KRDVPNMIVE ECGCA