PPA1, human recombinant | 7818

PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7818 | PPA1 human recombinant DataSheet

Biomolecule/Target: PPA1

Synonyms: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Alternates names: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061

Taglines: Catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions

NCBI Gene ID #: 4909

NCBI Gene Symbol: NTF4

Gene Source: Human

Accession #: P34130

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml.

Results: Specific activity is >28,000 pmoles/min/µg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35.2 kDa (313 aa, 1-289 aa + His Tag)

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A