Description
PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
7818 | PPA1 human recombinant DataSheet
Biomolecule/Target: PPA1
Synonyms: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061
Alternates names: Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061
Taglines: Catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions
NCBI Gene ID #: 4909
NCBI Gene Symbol: NTF4
Gene Source: Human
Accession #: P34130
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: The ED as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures was found in the range of 20-50 ng/ml.
Results: Specific activity is >28,000 pmoles/min/µg
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 35.2 kDa (313 aa, 1-289 aa + His Tag)
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: 0.5 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 200 mM NaCl and 2 mM DTT
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and store at 4°C for 1 week or 20°C for future use.
Amino acid sequence: N/A