PPM1F, human recombinant | 7810

PPM1F is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase can interact with Rho guanine nucleotide exchange factors (PIX), and thus block the effects of p21-activated kinase 1 (PAK), a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma (CAMK2G/CAMK-II) is found to be one of the substrates of this phosphatase. Recombinant human PPM1F protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7810 | PPM1F human recombinant DataSheet

Biomolecule/Target: PPM1F

Synonyms: Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2

Alternates names: Protein phosphatase 1F, CAMKP, CaMKPase, FEM-2, hFEM-2, POPX2

Taglines: A negative regulator of cell stress response pathways.

NCBI Gene ID #: 8601

NCBI Gene Symbol: OPG

Gene Source: Human

Accession #: O14789

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to neutralize the stimulation of U937 cells treated with 10 ng/ml of sRANKL.

Results: Specific activity is > 1200 unit/mg

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 52 kDa (477 aa, 1-454 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 150 mM NaCl and 1 mM DTT.

Reconstitution Instructions: Reconstitute in 5 mM Tris, pH 7.5 to a concentration of 0.1-1.0 mg/ml. The solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A