Description
Human neutrophil collagenase (HNC) has been purified from extracts of fresh and outdated buffy coats and from exudates of phorbol myristate acetate-stimulated neutrophils. The MMP-8 present in the starting material can either be latent or active, or have an app. relative molecular mass of 75-kDa and/or 58-kDa. The rather complex pattern of activation of the latent 58-kDa and 75-kDa species by trypsin, organomercurials and oxidants has been investigated. MMP-8 was shown to preferentially hydrolyze type I over type II, and type III collagens in solution and to be a glycoprotein that contains complex N-linked oligosaccharides leading to multiple forms of MMP-8 in SDS-PAGE. The action of endoglycosidase on the latent 58-kDa form produces 42/40-kDa species (Gao et al. 1992, Mallya et al. 1990). This indicates that MMP-8 is an N-linked, complex glycoprotein that appears to be glycosylated at multiple sites.
7784 | Pro-MMP-8 human DataSheet
Biomolecule/Target: N/A
Synonyms: Matrix metalloproteinase-13
Alternates names: Neutrophil collagenase (EC 3.4.24.34) (Matrix metalloproteinase-8) (MMP-8) (PMNL collagenase) (PMNL-CL)
Taglines: A Protein involved in breakdown of extracellular matrix
NCBI Gene ID #: 6352
NCBI Gene Symbol: TCP228
Gene Source: Human
Accession #: P13501
Recombinant: No
Source: Human neutrophil granulocytes (Buffy Coat)
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 40/42 kDa
Concentration: >100 mUnits/mg
Appearance: Liquid
Physical form description: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl; 1 M ZnCl; 0.05% Brij 35; 0,05% NaN
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 µg/µl. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or 20°c for future use.
Amino acid sequence: N/A