Pro-MMP-8, human | 7784

Human neutrophil collagenase (HNC) has been purified from extracts of fresh and outdated buffy coats and from exudates of phorbol myristate acetate-stimulated neutrophils. The MMP-8 present in the starting material can either be latent or active, or have an app. relative molecular mass of 75-kDa and/or 58-kDa. The rather complex pattern of activation of the latent 58-kDa and 75-kDa species by trypsin, organomercurials and oxidants has been investigated. MMP-8 was shown to preferentially hydrolyze type I over type II, and type III collagens in solution and to be a glycoprotein that contains complex N-linked oligosaccharides leading to multiple forms of MMP-8 in SDS-PAGE. The action of endoglycosidase on the latent 58-kDa form produces 42/40-kDa species (Gao et al. 1992, Mallya et al. 1990). This indicates that MMP-8 is an N-linked, complex glycoprotein that appears to be glycosylated at multiple sites.

7784 | Pro-MMP-8 human DataSheet

Biomolecule/Target: N/A

Synonyms: Matrix metalloproteinase-13

Alternates names: Neutrophil collagenase (EC 3.4.24.34) (Matrix metalloproteinase-8) (MMP-8) (PMNL collagenase) (PMNL-CL)

Taglines: A Protein involved in breakdown of extracellular matrix

NCBI Gene ID #: 6352

NCBI Gene Symbol: TCP228

Gene Source: Human

Accession #: P13501

Recombinant: No

Source: Human neutrophil granulocytes (Buffy Coat)

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 40/42 kDa

Concentration: >100 mUnits/mg

Appearance: Liquid

Physical form description: In 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl; 1 M ZnCl; 0.05% Brij 35; 0,05% NaN

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 µg/µl. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°c for future use.

Amino acid sequence: N/A