26
Pro-Urokinase, human recombinant | 7695
- SKU:
- 26-7695-GEN
- Availability:
- Usually shipped in 5 working days
Description
Urokinase or Urokinase-type plasminogen activator (uPA) is a serine protease (EC 3.4.21.73). It is secreted as a single-chain zymogen, pro-Urokinase, possessing little or no intrinsic enzymatic activity. The single chain zymogen is converted into the active two chain enzyme (tcuPA) by cleavage of the bond between Lys157 and Ile158. After activation, Urokinase specifically cleaves the proenzyme plasminogen to form the active enzyme plasmin. The active plasmin then catalyzes the breakdown of fibrin polymers of blood clots. Urokinase is involved in a number of biological functions including fibrinolysis, embryogenesis, cell migration, tissue remodeling, ovulation, and wound healing. Additionally, it is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.
7695 | Pro-Urokinase human recombinant DataSheet
Biomolecule/Target: Pro-Urokinase
Synonyms: Single chain Urokinase-type plasminogen activator (scuPA), Urokinase-type Plasminogen Activator uPA, PLAU.
Alternates names: SRPSOX
Taglines: After activation, forms Urokinase, which is a serine protease
NCBI Gene ID #: 58191
NCBI Gene Symbol: CXCL16
Gene Source: Human
Accession #: Q9H2A7
Recombinant: Yes
Source: E. coli.
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).
Activity (Specifications/test method): Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-100.0 ng/ml.
Biological activity: Determined by its ability to chemoattract human lymphocytes using a concentration range of 1.0-100.0 ng/ml.
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 10.1 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Amino acid sequence: NEGSVTGSCY CGKRISSDSP PSVQFMNRLR KHLRAYHRCL YYTRFQLLSW SVCGGNKDPW VQELMSCLDL KECGHAYSGI VAHQKHLLP