Procalcitonin, human recombinant | 4009

Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcitonin and katacalcin. Levels of unprocessed Procalcitonin rise significantly after bacterial infection, trauma or shock. Recombinant Human Procalcitonin produced in E.coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids with a molecular weight of 12,796 Daltons.

4009 | Procalcitonin human recombinant DataSheet

Biomolecule/Target: Procalcitonin

Synonyms: Procalcitonin, calcitonin, CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; MGC126648

Alternates names: AR, AREG, Colorectum cell-derived growth factor (CRDGF)

Taglines: The peptide precursor of calcitonin involved in calcium homeostasis

NCBI Gene ID #: 374

NCBI Gene Symbol: AREG

Gene Source: Human

Accession #: P15514

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

Biological activity: Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 for this effect is 5-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 11.3 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 5 mM Sodium phosphate, pH 7.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: SVRVEQVVKP PQNKTESENT SDKPKRKKKG GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK