Protein A/G/L | 6540

Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains 6x His-tag on the N-terminus with five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody. Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies.

6540 | Protein A/G/L DataSheet

Biomolecule/Target: N/A

Synonyms: Protein A/G/L

Alternates names: CD23 antigen, Fc-epsilon-RII, Lymphocyte IgE receptor, BLAST-2

Taglines: Genetically engineered protein ideal for purification of polyclonal or monoclonal IgG antibodies

NCBI Gene ID #: 9606

NCBI Gene Symbol: CD23

Gene Source: Human

Accession #: Q30218

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 96%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Measured by its ability to induce TNF-alpha production by human PBMCs.

Biological activity: Measured by its ability to induce TNF-alpha production by human PBMCs.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.2 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5X PBS, pH 8.0.

Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Amino acid sequence: MELQVSSGFV CNTCPEKWIN FQRKCYYFGK GTKQWVHARY ACDDMEGQLV SIHSPEEQDF LTKHASHTGS WIGLRNLDLK GEFIWVDGSH VDYSNWAPGE PTSRSQGEDC VMMRGSGRWN DAFCDRKLGA WVCDRLATCT PPASEGSAES MGPDSRPDPD GRLPTPSAPL HS