Prothrombin, Human Plasma | 7684

Prothrombin is a vitamin K-dependent plasma protein which is synthesized in the liver. Prior to secretion into plasma, prothrombin undergoes post-translational modification by a vitamin K-dependent carboxylase which converts ten specific glutamic acid residues to -carboxyglutamic acid (gla). Conversion to thrombin is a key step in the blood coagulation pathway and catalyzes the coagulation of fibrinogen. Clinically, cases of selective deficiency are rare, although, in cases of liver cirrhosis, prothrombin is decreased. During activation, prothrombin is cleaved at Arg271-Thr272 and at Arg320-Ser321 to a "pro" fragment (fragment 1.2) and thrombin, the latter of which is composed of two chains covalently linked by a disulfide bond. There is an additional thrombin feed-back cleavage at Arg284-Thr285 resulting in an additional 13 amino acids being removed from the mature thrombin “A” chain.

7684 | Prothrombin Human Plasma DataSheet

Biomolecule/Target: Prothrombin

Synonyms: Coagulation factor II

Alternates names: Coagulation factor II

Taglines: Catalyzes the coagulation of fibrinogen

NCBI Gene ID #: 20304

NCBI Gene Symbol: CCL5

Gene Source: Human

Accession #: P30882

Recombinant: No

Source: Human Plasma

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract murine T cell population using a concentration range of 1-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 72 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl, 0.1 M NaCl pH 7.4

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

Amino acid sequence: N/A