PRPS1, human recombinant | 7828

PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7828 | PRPS1 human recombinant DataSheet

Biomolecule/Target: PRPS1

Synonyms: Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1

Alternates names: Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1

Taglines: An enzyme that catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate

NCBI Gene ID #: 20304

NCBI Gene Symbol: CCL5

Gene Source: Human

Accession #: P30882

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract murine T cell population using a concentration range of 1-10 ng/ml.

Results: Specific activity: > 1 Units/ml

Binding Capacity: N/A

Unit Definition: One unit will catalyze the formation of 1.0 µmole of AMP from ATP and ribose-5-phosphate per minute at pH 7.6 at 37ºC.

Molecular Weight: 36.9 kDa (338 aa, 1-318 aa + His Tag)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1 mM DTT and 0.1 M NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.

Amino acid sequence: N/A