PTEN, human recombinant | 4838

PTEN, a tumor suppressor, has been implicated in a large number of human tumors and is conserved from humans to worms. Characterization of PTEN protein showed that it is a phosphatase that acts on proteins and on 3-phosphorylated phosphoinositides, and can therefore modulate signal-transduction pathways that involve lipid second messengers. Recent results indicate that at least part of its role is to regulate the activity of the serine/threonine kinase AKT/PKB, and thus influence cell survival signaling. Recombinant PTEN is purified by proprietary chromatographic techniques.

4838 | PTEN human recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Phosphatase and tensin homolog, Mutated in Multiple advanced cancers 1, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del,

Alternates names: Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1, BZS, MHAM, PTEN1, 10q23del,

Taglines: A tumour supressor protein

NCBI Gene ID #: 81780

NCBI Gene Symbol: CCL5

Gene Source: Human

Accession #: P50231

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): Not available at this time

Biological activity: Recombinant Rantes is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and murine T-cells at a concentration between 1-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80.0 kDa (GST tagged)

Concentration: 100 µg/ml

Appearance: Liquid

Physical form description: 100 µg/ml in 50 mM Tris-Acetate, pH 7.5, 150 mM NaCl, 0.1 mM EDTA, 10 mM glutathione, 25 mM DTT, 0.1 mM PMSF and 25% Glycerol.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A