PYCR1, human recombinant | 7368

PYCR1 is a universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP (+) in some cell types. It forms a homopolymer and localizes to the mitochondrion. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). Recombinant human PYCR1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7368 | PYCR1 human recombinant DataSheet

Biomolecule/Target: PYCR1

Synonyms: Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3

Alternates names: Pyrroline-5-carboxylate reductase 1, ARCL2B, P5C, P5CR, PIG45, PP222, PRO3

Taglines: A universal housekeeping enzyme that catalyzes the NAD (P) H-dependent conversion of pyrroline-5-carboxylate to proline

NCBI Gene ID #: 81780

NCBI Gene Symbol: CCL5

Gene Source: Human

Accession #: P50231

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Recombinant Rantes is fully biologically active when compared to standard. The activity is determined by the ability to chemoattract total human lymphocytes and murine T-cells at a concentration between 1-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 35.5 kDa (339 aa, 1-319 aa + His Tag).

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.5) containing 10% glycerol.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized product with sterile HO at a concentration of 0.1 mg’s/ml, which can be further diluted into other aqueous solutions.

Amino acid sequence: N/A